期刊论文

Study on Cloning and Transforming Soybean (Glycine max (L.) Merrill) of Methionine-Rich Protein Gene

中文

分子植物育种

1672-416X

2016

14

8

1930-1939

10.13271/j.mpb.014.001930

江西省新世纪学术与技术带头人项目(20030304) 苏州市科技局计划项目(SYN201410) 苏州科技学院科研基金(XKZ201505) 江苏高校优势学科建设工程等项目共同资助

本校为第一机构

为克隆高甲硫氨酸蛋白基因并导入大豆以提高大豆甲硫氨酸(Methionine, Met)含量,本研究首次报道利用同源克隆的方法从芦苇中克隆高甲硫氨酸蛋白基因,并利用农杆菌介导法将高甲硫氨酸蛋白基因导入大豆。基因克隆及测序结果表明该基因编码区420 bp,编码139个氨基酸,其中含有21个Met,Met含量达到15.11%。GUS组织化学染色、荧光定量PCR和测序结果表明,该基因已整合进大豆基因组中。8株转基因大豆叶片甲硫氨酸含量测定表明:各转基因植株甲硫氨酸含量均有不同程度提高,最高幅度达51.11%。本研究可为高必需氨基酸贮藏蛋白编码基因的克隆和转化及大豆品质改良及其功能研究提供借鉴。

To clone a methionine-rich protein gene and introduce it into soybean to improve the methionine content, this study firstly reported to clone the methionine-rich protein (MRP) encoding gene by homologous cloning method from reeds and then transferred it into soybean mediated by Agrobacterium tumefaciens. The results of gene cloning and sequencing demonstrated that the gene contained 420 bp, and encoded 139 amino acids which contained 21 Met and the Met content was up to 15.11 mol%. The results of GUS histochemical staining, fluorescence quantitative PCR and sequencing showed that the MRP gene ...