期刊论文

The Research of Zinc Finger Family Gene OsBBX24 in Response to Heat Stress in Rice

中文

分子植物育种

1672-416X

2017

15

6

2035-2041

10.13271/j.mpb.015.002035

国家自然科学基金资助项目（31301081） 科技部国家科技支撑计划项目（2014BAD01B04） 教育部博士点基金资助项目（20134320120009）共同资助

本校为第一机构

环境因子光和温度对植物生长发育起重要调控作用,OsBBX24基因属于光温信号途径重要的调控元件B-box（BBX）家族基因成员。OsBBX24基因启动子序列分析发现其含有HSE热胁迫相关元件,水稻基因芯片数据分析发现其表达受热诱导,实时定量PCR分析进一步证明OsBBX24基因受热胁迫表达上调。热胁迫处理发现OsBBX24-RNAi转基因水稻材料株系苗期的耐热性比野生型Kitaake要弱,且超氧化物歧化酶、过氧化物酶活性比野生型低,脯氨酸含量也低于野生型,丙二醛含量比野生型高;热激转录因子HsfA2a及热激蛋白Hsp16.9、Hsp60热胁迫处理后野生型中的表达水平比OsBBX24-RNAi株系高。由此证实,OsBBX24基因在水稻响应热胁迫...

The light and temperature of environmental factors play an important role in the regulation of plant growth and development. OsBBX24 is a member of the B-box (BBX) family which is an important regulatory element in the light and temperature signaling pathway. OsBBX24 gene promoter sequence analysis revealed that it contained heat stress element HSE. Rice microarray data analysis showed that its expression was induced by heat and real-time quantitative PCR analysis showed that OsBBX24 gene was up-regulated by heat stress. Heat stress treatment found that the heat resistance of OsBBX24-RNAi tran...