期刊论文

中文

分子植物育种

1672-416X

2018

16

1

82-88

10.13271/j.mpb.016.000082

31270335:国家自然科学基金 31501367:国家自然科学基金 2014DFA32160:国家国际科技合作专项基金

本校为第一机构

黄花蒿中含有大量的香气类物质,在食品和医药行业有广泛的应用前景,但对其生物合成的分子机理研究非常欠缺。本研究采用SMART-RACE-PCR技术从黄花蒿花组织差异文库中获得了1个新的双键还原酶编码基因AaDBR3,其cDNA序列全长为1 279 bp,开放阅读框(ORF)长度为1 038 bp编码345氨基酸,蛋白分子量为38.46 kD,等电点(pI)为6.05。通过生物信息学的分析,AaDBR3属于中链脱氢酶超级家族中leukotriene B4 dehydrogenases类酶。系统发育进化树结果表明,该基因与烟草的烯丙醇脱氢酶(NtADH, GenBank: BAA89423.1)的同源性最高,相似性为77%。体外酶研究发现,重组蛋白酶能催化3-壬烯-2-酮向2-壬酮的转化,表明其属...

Artemisia annua contains a large amount of aroma compounds, which has a wide range of potential applications in the food and pharmaceutical industry, but the molecular mechanism of biosynthesis is poorly understood. In this study, a novel double bond reductase gene AaDBR3 was obtained from the differentially expressed gene library established using flowers of A. annua by SMART-RACE-PCR. The full-length cDNA of AaDBR3 was 1 279 bp, with a open read frame (ORF) of 1 038 bp, encoding 345 amino acid. The molecular weight of the protein was 38.46 kD, and the pI was 6.05. The bioinformatic analysis ...