期刊论文

Polymerization of Pi9 and Pi49 Loci by Marker Assisted Selection to Improve Blast Resistance of Dual-purpose Genic Sterile Rice Chuang 5S

中文

分子植物育种

1672-416X

2018

16

22

7372-7379

10.13271/j.mpb.016.007372

2016YFD0101100:国家重点研发计划 2015NK1001-1:湖南省科技重大专项 31171834:国家自然科学基金

本校为第一机构

为了改良水稻两系不育系创5S的稻瘟病抗性,分别以携带稻瘟病抗性基因Pi9位点（Pi9/Pigm/Pi2-1）的75-1-127、谷梅4号和天津野生稻以及携带Pi49位点的魔王谷为基因供体,通过回交转育和分子标记辅助选择技术,育成了包含单一抗性位点的创5S抗病单基因系;在此基础上,采用聚合杂交和分子标记辅助选择技术,培育了包含两个抗性位点的创5S抗病双基因聚合改良系。结果表明：携带单一抗性位点的改良不育系稻瘟病抗性显著增强,苗期发病级数显著低于受体亲本创5S;聚合2个抗性位点的改良系,较携带单一抗性位点的不育系稻瘟病抗性进一步增强、抗谱拓宽;部分改良不育系的株高、穗长、有效穗数、每穗总粒数、柱头...

In order to improve the resistance to rice blast of the dual-purpose genic sterile rice cultivar Chuang 5S （C5S）, the broad-spectrum resistance genes located on the Pi9 loci, which were harbored in the donor parents of 75-1-127 （75, Pi9）, GuMei 4 （GM4, Pigm） and Tianjinyeshengdao （TY, Pi2-1） were introgressed into C5S by successive backcross and molecular marker assisted selection technology. Thus, a series of C5S resistant single gene lines containing single resistance loci were bred. Based on this, polymeric hybridization and molecula...