期刊论文

Effects of Drought and Rehydration on Proline Accumulation and SiP5CR Gene Expression from Foxtail Millet (Setaria italica (L.) Beauv)

中文

分子植物育种

1672-416X

2018

16

22

7460-7465

10.13271/j.mpb.016.007460

2014QNM29:山东省农业科学院青年科研基金项目 CARS-06-13:现代农业产业技术体系建设专项 5-A19:现代农业产业技术体系建设专项 CXGC2016A02:创新工程项目

本校为第一机构

脯氨酸在植物对干旱胁迫应答中起重要的渗透调节作用,增加植物体内脯氨酸含量可以提高植物抗旱性,吡咯啉-5-羧酸还原酶(P5CR)是合成脯氨酸的重要还原酶。为探讨干旱对谷子脯氨酸含量及脯氨酸代谢关键基因的影响,本研究以抗旱性突出的谷子品种‘济谷16'为材料,测定了其地上部分在干旱和复水条件下脯氨酸含量,结果表明脯氨酸含量随胁迫程度的增强而增加,复水后下降。经PCR扩增获得SiP5CR基因全长cDNA序列,序列分析表明SiP5CR基因长度为852 bp,编码283个氨基酸(GenBank登录号: MG833228),含有P5CR保守域,与玉米和高粱亲缘关系最近,亲缘关系最远的是大豆和豇豆。利用荧光定量RT-qPCR分析了该基因在干...

Proline plays an important role of osmotic regulation in plant responses against drought stress. Increasing proline content accumulated in plants can improve plant drought tolerance. The pyrroline-5-carboxylic acid reductase (P5CR) is an important reductase in proline synthesis. To investigate effects of drought on proline content and key genes of proline metabolism in foxtail millet, the foxtail millet (Setaria italica L.) variety, 'Jigu 16', with an outstanding ability of drought stress tolerance, was used as test material to determine the proline content in the aboveground part under drough...