期刊论文

Expression of BnDGAT1 and BnPDAT1 Genes in the Development of Rape with Different Oil Content

中文

分子植物育种

1672-416X

2019

17

22

7320-7325

10.13271/j.mpb.017.007320

国家重点基础研究发展计划(973计划)项目(2015CB150206 SQ2018ZD080068 SYKB201701)资助。

本校为第一机构

油菜含油量主要受BnDGAT1和BnPDAT1基因调控,本研究以3组不同含油梯度的9个甘蓝型油菜为材料,利用定量PCR技术研究其在全生育期表达情况,以期从中找出导致含油量差异的主要基因。结果表明:BnDGAT1表达量在营养生长期时先上升后下降,在种子中表达量最高,在叶片、角果皮和种子中随含油量变化而变化;BnDGAT1在叶片、角果皮和种子中随含油量增加而增加;除花期外,BnDGAT1基因表达量和含油量的相关性均达极显著水平。BnPDAT1表达量在全生育期表达稳定;在幼苗期、21 d和35 d种子中达显著水平,在蕾薹期、35 d的角果皮、28 d种子中相关性均达显著水平。在叶片、角果皮和种子中,BnDGAT1的相关性高于BnPDAT...

The oil content is regulated by BnDGAT1 and BnPDAT1 in rapeseed. In order to find out the key gene which control lipid synthesis mainly in different growth stages, three groups of nine Brassica napus with different oil content were used as raw materials to study expression regular in the whole development stage by RT-PCR. The results showed that the expression of BnDGAT1 increased at first, and then decreased during development period, until reached the highest level in seeds. The expression changed with the oil content in leaves, pericarp and seeds. There has highly significant difference bet...