期刊论文

Determination of Ramie (Boehmeria nivea) Genome Size by Flow Cytometry

中文

分子植物育种

1672-416X

2019

17

1

99-103

10.13271/j.mpb.017.000099

国家自然科学青年基金（31401432）资助；

本校为第一机构

苎麻属于荨麻科苎麻属,是目前非常重要的纺织原料,有着广泛的开发前景。为了更好地研究苎麻基因组信息,拟通过流式细胞术测定苎麻基因组大小。本研究以水稻(Oryza sativa)日本晴为内标,湘苎3号为待测样品,分别分离水稻和苎麻叶片细胞的细胞核,PI染色,用流式细胞仪对水稻和苎麻样品的PI发射荧光强度进行测定。通过比较水稻和苎麻样品的荧光强度的倍数关系,计算得到苎麻基因组大小,并采用外标法和内标法同时进行测定和计算。以水稻日本晴为外标,苎麻3号为待测样品,测得苎麻基因组大小为332.5 Mb,而用内标法得到的苎麻基因组大小为300.5 Mb,相比之下内标法测定的变异系数更低,更为准确。苎麻基因组...

Boehmeria nivea (ramie), which belongs to Urticaceae in Boehmeria, is an important textile material at present with a broad development prospects. In order to better study the genomic information of ramie, the genomic size of ramie was determined by flow cytometry. One of the ramie variants Xiangzhu 3 was used as the material and Oryza sativa Japonica as reference, and the nucleus of the leaf cells of both species were isolated and stained with propidium iodide (PI), then the PI emission fluorescence intensity was measured by flow cytometry. Ramie's genome size was calculated according to the ...