期刊论文

中文

分子植物育种

1672-416X

2019

17

10

3253-3258

10.13271/j.mpb.017.003253

湖南省教育厅资助科研项目（14K045）资助；

本校为第一机构

WRKY是一类广泛参与高等植物各种抗逆调控活动的转录因子,可以通过激活下游相关信号转导途径调节自身的应激反应,进而增强植物抗逆性。本研究通过RNA-Seq从马铃薯(Solanum tuberosum)中筛选出一个晚疫病菌诱导WRKY转录因子基因StWRKY。采用RT-PCR技术获得该基因CDS全长,并对其进行序列分析及结构功能预测。根据StWRKY蛋白序列进行同源性搜索,得到与其蛋白序列相似度较高的其他物种的蛋白序列,使用MEGA7软件对StWRKY蛋白序列及其同源序列进行多序列比对分析并构建了系统进化树。利用在线工具ProtParam对StWRKY进行氨基酸理化性质分析;利用SMART在线工具进行蛋白序列分析;利用SWISS-MODEL在线工具...

WRKY is a kind of transcriptional factor that widely participates in the regulation of various stress resistant activities of higher plants, which can regulate its own stress response by activating downstream related signal transduction pathway to enhance plant stress resistance. In this study, a WRKY transcription factor gene StWRKY induced by Phytophthora infestans was screened from Solanum tuberosum by RNA-seq. The full-length CDS of the gene was obtained by RT-PCR, and the sequence analysis and structural function prediction were carried ou...