期刊论文

中文

分子植物育种

1672-416X

2019

17

20

6673-6679

10.13271/j.mpb.017.006673

国家自然基金面上项目（31571707）资助；

本校为第一机构

分泌信号肽是生物体内的一类重要功能分子。通过生物信息学分析,揭示甘蓝型油菜BnaLCR78基因编码一个由78个氨基酸组成、N端包含分泌信号肽且C端富含半胱氨酸的蛋白,属于富含半胱氨酸的分泌信号肽。为进一步研究该基因的功能,借助农杆菌介导的甘蓝型油菜下胚轴遗传转化方法,利用CRISPR/Cas9系统对甘蓝型油菜BnaLCR78基因进行编辑,实现定向敲除。本实验共计使用1 200个外植体,经除草剂草铵膦的抗性筛选及对213株抗性苗基因组DNA的PCR检测,获得了26株转基因油菜,转化率约为2.17%,但在T0代并未获得发生了目标基因被编辑的植株。幸运地是从T0自交获得的T1植株中获得基因被编辑了的植株。通过扩增T1代...

Secretory signal peptides are an important class of functional molecules in living organisms. Through bioinformatics analysis, it was revealed that the BnaLCR78 gene of Brassica napus L. encodes a protein consisting of 78 amino acids with a N-terminal secretion signal peptide and a C-terminal cysteine-rich peptile, and belonged to cysteine-rich secretion signal peptide. In order to further studying the function of this gene, gene target editing of the BnaLCR78 gene of Brassica napus L. using CRISPR/Cas9 system was achieved via the Agrobacteriummediated transformation of Brassica napus hypocoty...