期刊论文

Functional Study of Arabidopsis thaliana SB10 Gene in Salt Tolerance

中文

分子植物育种

1672-416X

2019

17

1

92-98

10.13271/j.mpb.017.000092

国家自然基金面上项目（31771838）资助；

本校为第一机构

为了研究拟南芥SB10基因在耐盐中的功能,我们利用CRISPR/Cas9技术构建载体敲除SB10基因,对转基因植株进行筛选成功获得SB10基因沉默的突变体材料。本研究通过观察突变体根长及萌发率等表型,测定突变体植株脯氨酸含量相关的抗逆生理指标,研究拟南芥SB10基因在耐盐中的功能。结果显示:NaCl胁迫下,sb10突变体的根长和萌发率明显高于野生型拟南芥,sb10突变体拟南芥植物体内脯氨酸含量明显高于野生型拟南芥。说明SB10基因功能的缺失可提高拟南芥植株的耐盐能力。该研究为SB10基因参与拟南芥植株耐盐中的分子作用途径及分析提供线索。

In order to stud y the function of Arabidopsis thaliana SB10 gene in salt tolerance, we uses CRISPR/Cas9 technology to construct a vector to knock out the SB10 gene and screened the transgenic plants to obtain the mutant material of SB10 gene silencing. In this paper, through the observation of mutant phenotype, such as root length and germination rate, the physiological indexes related to proline content in mutant plants were determined, so as to investigate the function of Arabidopsis thaliana SB10 gene in salt tolerance. The results showed that under NaCl stress, the root length and germina...