期刊论文

Regulation of CYP83A1 Gene Expression in Cabbage Seedling by Exogenous Substances and Its Bioinformatics Analysis

中文

分子植物育种

1672-416X

2019

17

24

7996-8004

10.13271/j.mpb.017.007996

31772325:国家自然科学基金 2017JJ2118:湖南省自然科学基金 2018JJ3217:湖南省自然科学基金 16A093:湖南省教育厅重点项目 2018NK2022:湖南省重点研发计划 17QN33:湖南农业大学青年项目

本校为第一机构

为深入探索CYP83A1介导外源物质对芥蓝幼苗中萝卜硫素合成调控的功能,以芥蓝BOK92为材料,采用石英砂栽培,研究了外源氯化钠(NaCl)、茉莉酸甲酯(MeJA)和亚硒酸钠(Na2SeO3)对芥蓝幼苗萝卜硫素含量及BoCY P83A1基因表达的影响,同时对BoCYP83A1蛋白进行了生物信息学分析.结果 表明,50 μmol/L NaC1处理诱导芥蓝幼苗中BoCY P83A1基因表达下调显著,抑制了萝卜硫苷的生物合成,造成萝卜硫素含量显著下降;40 μmol/L MeJA处理下该基因表达上调显著,促进了萝卜硫苷的生物合成,萝卜硫素含量显著升高,这表明BoCY P83A1基因在萝卜硫苷生物合成和萝卜硫素积累过程中起重要的调控作用.在100 μmol/L Na2SeO3处理...

In order to explore the functio n of gene CYP83A1 involving sulforaphane metabolism regulation induced exogenous substance in Chinese Broccoli seeding, the experiment was carried out to study the effects of exogenous treatment (NaCl, MeJA and Na_2SeO_3)on CYP83A1 gene expression and sulforaphane content in Chinese broccoli (Brassica oleracea var. alboglabra 'BOK92')seedlings with sand culture. The results showed that expressions of CYP83A1 gene were down-regulated under 50 µmol/L NaCl treatment, then glucoraphanin and sulforaphane content were...