期刊论文

中文

分子植物育种

1672-416X

2019

17

15

5163-5170

10.13271/j.mpb.017.005163

广东省科技计划项目(2014A020208071) 清远市科技计划项目(2015A014)共同资助.

本校为第一机构

本研究对32份金线莲(Anectochilus roxburhii)种质资源的四个DNA条形码(ITS, rbcL, psbA-trnH, matK)基因进行PCR扩增及测序,从基因库下载了金线莲及其3种主要混伪品的29条序列,分析比较各序列的差异位点、信息位点、GC含量等,并进行种内和种间的遗传距离计算,构建基于K2P模型的NJ系统发育树,综合比较不同序列的鉴定能力,最终从四个DNA条形码候选序列中筛选出可用于金线莲资源鉴定的通用序列。结果显示,ITS和psbA-trnH基因序列的种内、种间的变异位点差异和遗传距离差异较大,相比rbcL和matK序列具有显著优势,且ITS基因不仅能够快速而准确区分金线莲及其混伪品,而且能够在种内水平上区分金线莲的...

In this study, four DNA barcode (ITS, rbcL, psbA-trnH, matK) genes from 32 Anoectochilus roxburhii germplasm resources were amplified by PCR and sequenced. We downloaded 29 sequences of Anoectochilus roxburghii and its three main mixed pseudo-products from gene bank, to analyze and compare the difference sites, information sites and GC content of each sequence. Then we calculated the genetic distance between species and species, constructed NJ phylogenetic tree based on K2P model, and comprehensively compared the identification ability of different sequences. Finally, the general sequence that...