期刊论文

Cloning and Expression Analysis under Abiotic Stresses of stuProt1.2 Genes in Potato

中文

分子植物育种

1672-416X

2019

17

19

6243-6255

10.13271/j.mpb.017.006243

国家自然科学基金项目(31371683) 国家重点研发计划(2017YFD0101905) 国家马铃薯产业技术体系-长沙综合试验站(CARS-09-ES16)共同资助.

本校为第一机构

为了探究马铃薯脯氨酸转运蛋白在逆境下的功能,以马铃薯GS393 (Solanumc ommers onii-LZ3.4-Wisconsin,United States)为试验材料,采用PCR的方法,克隆得到2个脯氨酸转运蛋白基因分别命名为s tuProT1和stuPro T2,它们的开放阅读框长度分别为504bp和600bp,分别编码189个和199个氨基酸.系统进化树分析结果表明,这2个蛋白和番茄ProT蛋白具有高度同源性.实时荧光定量PCR表明,stuPro T1和s tuPr T2基因在GS393不同组织(根,茎,叶,匍匐茎和块茎)中均有表达,其中根和茎的表达量较高.qPCR分析这2个基因在不同激素、重金属、低温、干旱和盐胁迫下的表达情况.分析结果表明:ABA抑制stuProT1和stuPr T2基因表达...

To identified the function of potato proline transporter under stress conditions, potato GS393 (Solanum commersonii-LZ3.4-Wisconsin, United States) was used as the experimental material, and two proline transporter genes were cloned for stuProT1 and stuProT2 by PCR. stuProT1 open reading frame is 504 bp in length and encodes 189 amino acids; stuProT2 open reading frame is 600 bp in length and encodes 199 amino acids. Phylogenetic tree analysis showed that the two proteins and tomato ProT protein have high homology. Real-time PCR showed that stu...