期刊论文

中文

分子植物育种

1672-416X

2019

17

5

1385-1392

10.13271/j.mpb.017.001385

2014AA10A604:国家高技术研究发展计划（863计划） 2017XC09:湖南农业科技创新资金共同资助

本校为第一机构

三结构域多铜氧化酶是生物体内非常重要的金属氧化酶,对机体生长和发育具有重要影响。为了系统地了解水稻三结构域多铜氧化酶基因家族的基本情况,本研究运用生物信息学方法对其成员进行了预测和分析;研究发现,以拟南芥三结构域多铜氧化酶基因At5g21105为靶序列,利用BlastP为工具在水稻基因组中共搜索到38个同源基因;这些基因的蛋白产物都依次含有Cu-oxidase3、Cu-oxidase和Cu-oxidase2三种结构域,各结构域中含有铜离子结合位点,二级结构具有α-螺旋和β-转角等基本特征,特推断它们都属于三结构域多铜氧化酶家族成员;进一步地,根据同源性程度将水稻多铜氧化酶基因家族成员划分为ClassⅠ和ClassⅡ...

Three-domain multi-copper oxidases, a very important metal oxidase in organism, are essential for the growth and development of organism. In order to understand the basic situation of multi-copper oxidase gene family in rice, in this study, bioinformatics methods were used to predict and analyze its family members. By performing a program of BlastP with a target sequence of At5g21105 encoding a three-domain multi-copper oxidase, 38 homologous proteins were identified from rice genome. Furthermore, these proteins products of these genes were pre...