期刊论文

Effects of NaCl Stress on Physiology and Biochemistry of Potato Plantlets in Tissue Culture

中文

分子植物育种

1672-416X

2020

18

14

4754-4761

10.13271/j.mpb.018.004754

国家重点研发计划(2017YFD0101905) 国家自然科学基金项目(31371683)共同资助。

本校为第一机构

为探究盐胁迫对马铃薯生理的影响,以耐盐品种‘陇薯5号'组培苗为试材,采取添加0 mmol/L,100 mmol/L,300 mmol/L,500 mmol/L,700 mmol/L和900 mmol/L NaCl的MS培养基模拟盐胁迫的方法,筛选盐胁迫生理测定的适宜盐浓度,并测定适宜胁迫浓度下(0 mmol/L NaCl为对照)、胁迫不同时间(0, 20 h, 24 h,48 h, 72 h和96 h)的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)等抗氧化酶的活性,膜脂过氧化物质丙二醛(MDA)含量,脯氨酸(PRO)、可溶性蛋白和可溶性糖等可溶性物质的含量。结果表明,这7项参数可以作为马铃薯耐盐性鉴定的生理指标,CAT活性与其他指标呈负相关,其他各指标彼此之间均显著或极显...

In order to investigate the effect of salt stress on potato physiology, ’Longshu No.5’, a salt-tolerant seeding,was used as the test material. The MS medium supplemented with 0 mmol/L, 100 mmol/L, 300 mmol/L, 500 mmol/L,700 mmol/L and 900 mmol/L NaCl was used to simulate the salt stress, screening the suitable salt concentration for salt stress. Seven indicators related to antioxidant, membrane peroxidation and soluble substance regulation pathways were determined at 6 stress time points(0, 20 h, 24 h, 48 h, 72 h and 96 h) under suitable salt...