期刊论文

Construction of CRISPR/Cas9 Editing Vector of Brassica napus BnaABI4 Gene and Transformation of Brassica napus

中文

分子植物育种

1672-416X

2020

18

4

1144-1151

10.13271/j.mpb.018.001144

国家自然基金面上项目(31771838) 湖南文理学院科研项目(19YB24)共同资助。

本校为第一机构

ABI4 (Abscisic acid-insensitive 4)是ABA响应途径中重要的转录因子.本研究以甘蓝型油菜全基因组数据库为基础,借助拟南芥ABI4蛋白的氨基酸序列,通过序列比对,获得了甘蓝型油菜的2个BnaA BI4,分别命名为BnaA BI4-A (GenBank登录号为BnaA03g18970D)和BnaABI4-C (GenBank登录号为BnaC03g725-10D).在这两个基因的CDS序列上寻找一段长度为20 bp、序列相同的片段作为基因编辑的目标位点,构建CRISPR/Cas9基因编辑载体pCAMBIA 1300-sgRNA/Cas9-BnaABI4;借助根癌农杆菌介导的油菜下胚轴遗传转化法,成功将BnaA I4的基因编辑表达框转入甘蓝型油菜‘湘油15’中,共获得转基因植株13株,这将为深入研究甘蓝型...

ABI4 (Abscisic acid-insensitive 4) is an important transcription factor in ABA response pathway. In this study, based on the whole genome database of Brassica napus, two BnaABI4 of Brassica napus were obtained by amino acid sequence aligment of Arabidopsis ABI4 protein, and named BnaA BI4-A (GenBank accession number BnaA03g18970D) and BnaABI4-C (GenBank accession number BnaC03g72510D) respectively. In the CDS of the two genes, a 20 bp fragment with the same sequence was found as the target site for gene editing, and the CRISPR/cas9 gene editing vector pCAMBIA1300-sgRNA/Cas9-BnaABI4 was constru...