期刊论文

Isolation and Purification of Protoplasts from Mesophyll Tissues of Macleaya cordata

中文

分子植物育种

1672-416X

2020

18

10

3341-3347

10.13271/j.mpb.018.003341

国家重点研发计划资助项目(2017YFD0501500) 现代农业产业技术体系建设专项资金(CARS-21)共同资助。

本校为第一机构

博落回(Macleaya cordata)是一种罂粟科博落回属的多年生草本植物,其体内的血根碱(sanguinarine,SAN)具有抗炎、调控肠道菌群和促进动物生长的作用,因此长期以此作为饲用抗生素的天然源替代品在全世界70个国家和地区广泛销售。随着全世界越来越多的国家限用甚至禁止饲用抗生素,博落回资源的需求逐年增加。因此,通过育种等方法来提高博落回中血根碱的含量具有十分重要的意义。而利用原生质体技术进行品种改良是一个快速高效的方法,其中制备原生质体是第一步。本研究以博落回无菌苗叶片为材料,进行了原生质体分离和纯化。在一定条件下,通过比较不同的酶解渗透压、温度、时间和pH值,得到最适甘露醇...

Macleaya cordata is a perennial herb of the genus Papaveraceae.It contains sanguinarine (SAN),which has anti-inflammatory properties,regulates intestinal flora and promotes animal growth.It has long been a natural source substitute for feed antibiotics,and widely used as a natural source substitute for feed antibiotics in 70 regions around the world.As more and more countries around the world restrict or even ban the use of feed antibiotics,the demand for resources is increasing year by year.Therefore,it is of great significance to improve the ...