期刊论文

Isolation and Identification of the Pathogens Causing Blueberry Root Rot and ToxicityMeasurement

中文

分子植物育种

1672-416X

2020

18

14

4687-4691

10.13271/j.mpb.018.004687

湖南省高校科技创新平台建设经费(17K046) 湖南省大学生科技创新项目经费(XCX18051)共同资助。

本校为第一机构

为了解浙江蓝莓根部腐烂的原因及病原菌种类,从而控制该病在蓝莓生产上的扩散。本研究从浙江蓝莓种植区采集了蓝莓根腐病病样,通过分离纯化获得菌落形态相似的3个菌株,经柯赫氏法则验证、形态学观察、选取真菌通用引物ITS4/ITS5扩增菌株的rDNA-ITS序列并进行测序,确定分离到的菌株均为尖孢镰刀菌(Fusarium oxysporum)。采用菌丝生长速率法研究7种不同的杀菌剂对F.oxysporum的毒力作用,测定各杀菌剂的有效中浓度(EC_(50)),发现咪鲜胺和多菌灵的抑制作用显著,EC_(50)分别为0.071 59 mg/L和2.832 9 mg/L。通过对蓝莓根腐病的病原菌的种类鉴定,明确了该病的病原菌,并进行了室内药剂毒力测定,本试验结...

In order to find out the cause of root rot and pathogen species of blueberry in Zhejiang and control the spread of the disease in blueberry production. Blueberry root rot disease samples were collected from blueberry growing area in Zhejiang province, and three strains with similar colony morphology were isolated and purified.After validation by kirschner’s rule, morphological observation, rDNA-ITS sequences of the fungus general primer ITS4/ITS5 amplification were selected and sequenced, and it was confirmed that all strains isolated were Fus...