以水稻(Oryza sativa L. )的胚性悬浮细胞和空心莲子草[Alternanthera philoxeroides (Mart.) Griseb ]的悬浮细胞为材料,研究了两种原生质体电融合的条件.结果表明:在交流电场为100V/cm,直流脉冲电场为1.5kV/cm,脉冲幅宽为40μs,脉冲间隔为0.5s,脉冲次数为2次,电融合液组成为10%甘露醇和0.4mmol/LCaCl2时,原生质体的融合频率达到24.6%.
摘要(英文)：
The study was attempted to find the condition suitable for the electrofusion of protoplasts isolated from cell suspension cultures of Oryza sativa L. and Alternanthera philoxeroides (Mart.) Griseb. It showed that the fusion frequency of protoplasts reached 24.6% with the following parameters: alternate current (AC) 100V/cm , direct current (DC) 1.5kV/cm , DC pulse width (PW) 40μs , DC pulse interval 0.5 s, Number of DC pulse 2 , 10% mannit...
