期刊论文

中文

中国农学通报

1000-6850

2008

24

5

83-86

国家自然科学基金项目“植物激素、植酸与杂交水稻籽粒灌浆的关系及其调控研究”（30670190）；

本校为第一机构

为了适合于中花11的转化,笔者在愈伤组织预培养时间、浸染时间和凝固剂等方面改进了一种针对日本晴的水稻快速转化方法。将消毒后的中花11成熟种子在含有2.0mg/L 2,4-D的培养基中培养8d,不用继代愈伤组织,用含有GFP报告基因的根癌农杆菌直接浸染诱导的愈伤组织,经3d共培养和14d的筛选培养后,直接用新生的愈伤组织进行再分化,最终在40d内就获得了水稻转基因植株,转化效率和再生效率分别达到了71.3%和57%。从而证明了利用农杆菌直接浸染水稻早期愈伤的快速转化是切实可行的。

For making it adapt to Zhonghua11 transformation, researchers improve a high-speed transformation method adapting to the japonica rice cultivar ＇Nipponbare＇ on seeds pre-cuhure, time of infection and choosing gel. The sterilized seeds of Zhonghual 1 were pre-cultured on medium with. 2.0 mg/L 2,4-D, and the 8 days old calli were immediately infected by Agrobacterium containing GFP.ABD2 but not undergo subculture. After 3 days co-culture and 14 days selection, Proliferating calli were directly differentiated on regeneration medium. Successfully...