期刊论文

Comparison of Pollen Viability Determining Methods for Miscanthus sinensis Anderss

中文

中国农学通报

1000-6850

2009

25

24

147-150

美国Mendel Bioteehnology,Inc.合作项目.

本校为第一机构

采用离体萌发法、FDA染色法和I2-KI染色法测定芒离体花粉的生活力,并对测定效果进行比较.结果表明,利用离体萌发法测得芒离体花粉的平均初始萌发率为82.6%,并且花粉萌发和生长很快,5 min即开始萌发,培养30min后花粉管平均长度达到了145.77μm,但花粉的平均萌发率下降很快.室温保存90min的花粉其萌发率已下降至3.0%.利用FDA染色法和I2-KI染色法测定的芒花粉初始生活力与离体萌发法的结果基本一致,分别为84.6%和86.6%,但这两种染色法在跟踪测定芒离体花粉的生活力时易误判,不适合用于跟踪测定花粉生活力的变化.利用离体萌发法能够准确有效地测定芒花粉生活力的变化规律,而FDA染色法和I2-KI染色法适...

The pollen viability of Miscanthus sinensis was determined by in vitro pollen germination method, I2-KI staining method and FDA staining method, respectively. These three methods were analyzed to find their advantages, disadvantages and effectiveness. The results showed that with the in vitro pollen germination method, the pollens germinated in 5 minutes and the pollen tubes reached an average length of 145.77 μm in 30 minutes. The average initial pollen germinating rate was 82.6%, but it was decreased sharply to 3.0% when the pollens were sto...