期刊论文

中文

中国农学通报

1000-6850

2012

28

34

268-273

10.3969/j.issn.1000-6850.2012.34.046

湖南农业大学博士后基金“富铜富锌微生物的筛选及在环境重金属处理中的应用”（71709）；

本校为第一机构

为了筛选并鉴定一株耐铜富铜菌株并用于环境中重金属铜的清除,从有一定圈养时间的生猪养殖场周围土壤中,运用微生物筛选的方法广泛分离筛选耐铜微生物,再反复分离纯化筛选目标菌株,对其进行了形态和分子鉴定,在400倍光学显微镜及扫描电镜下进行形态特征观察,并将基于5.8SrDNA通用引物扩增的测序片段在GenBank数据库中使用BLAST工具进行比对。得到10株不同耐铜浓度的真菌菌株,其中耐性最高的菌株A4,其耐铜浓度达到940mg/L。A4菌在察氏琼脂培养基上生长迅速,综合确定该菌株为烟曲霉(Aspergillus fumigatus)。菌株A4对茶园土壤中的铜有一定吸附作用。

The aims were to screen and identify a microorganism strain with absorbing and resisting to copper and to use it removing heavy metal copper in the environment.By using microorganism screening and separating methods,the copper-resisting microorganism were widely obtained from the soil around pig farms with a long captive time.The target microorganism strains were screened and purified repeatedly.Their morphology and molecular identification were analyzed and the microorganism strains were applied to absorb heavy metal copper in tea garden soil.Morphological characteristics were observed under ...