To study the gene function of sb3 and sb6 containing two MATH domains, we need to obtain the double mutants of these two closely linked genes. CRISPR/Cas9 gene editing technology was used to create special double knockout mutants of sb3 and sb6 genes in Arabidopsis thaliana Col-0., 10 T_0 transgenic plants were obtained by PCR and Singer Sequencing. Among them, 5 transgenic plants had no editing in targetsequence. In the other five transgenic plants, the target sequence of sb3 gene was edited, but the targetsequence of sb6 gene was edited only in 2 transgenic plants. So far, the double-knockou...
