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Surface plasmon resonance immunosensor for fast, highly sensitive, and in situ detection of the magnetic nanoparticles-enriched Salmonella enteritidis

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成果类型:
期刊论文
作者:
Liu, Xia*;Hu, Yuxin;Zheng, Shu;Liu, Yan;He, Zao;...
通讯作者:
Liu, Xia
作者机构:
[Liu, Yan; He, Zao; Hu, Yuxin; Luo, Fang; Liu, Xia; Zheng, Shu] Hunan Agr Univ, Coll Food Sci & Technol, Hunan Prov Key Lab Food Sci & Biotechnol, Hunan Coinnovat Ctr Utilizat Bot Funct Ingredient, Changsha 410128, Hunan, Peoples R China.
[Liu, Xia] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Liu, Xia] H
Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Surface plasmon resonance immunosensor;Salmonella enteritidis detection;Immune magnetic nanoparticles separation;Sandwich assay
期刊:
Sensors and Actuators B-Chemical
ISSN:
0925-4005
年:
2016
卷:
230
页码:
191-198
基金类别:
Special Fund for Agro-Scientific Research in the public interest [201303084]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2015JJ3077]; "1515"talent cultivation plan of Hunan Agricultural University
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
A highly sensitive surface plasmon resonance (SPR) immunosensor combining antibody-functionalized Fe3O4magnetic nanoparticles (immunoMNPs) separation is reported for rapid detection of Salmonella enteritidis (S. enteritidis) based on the sandwich immunoassay. Here, immunoMNPs simultaneously served not only as "vehicles" for rapid delivery of analyte from a sample to the sensor surface, but also as labels increasing the measured refractive index changes associated with the binding of analyte. S. enteritidis can be detected at the concentration as low as 14 cfu/mL with a good linear signal range...

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