Special Fund for Agro-Scientific Research in the public interest [201303084]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2015JJ3077]; "1515"talent cultivation plan of Hunan Agricultural University
机构署名:
本校为第一且通讯机构
院系归属:
食品科学技术学院
摘要:
A highly sensitive surface plasmon resonance (SPR) immunosensor combining antibody-functionalized Fe3O4magnetic nanoparticles (immunoMNPs) separation is reported for rapid detection of Salmonella enteritidis (S. enteritidis) based on the sandwich immunoassay. Here, immunoMNPs simultaneously served not only as "vehicles" for rapid delivery of analyte from a sample to the sensor surface, but also as labels increasing the measured refractive index changes associated with the binding of analyte. S. enteritidis can be detected at the concentration as low as 14 cfu/mL with a good linear signal range...