期刊论文

Extrinsic Fluorescent Probe Analysis for Interactions Between Transgenic Insect-resistant Protein Cry1Ac and Surfactants by Pyrene

Liang Yunshan;Wang Zhi

中文

高等学校化学学报

0251-0790

2018

39

5

883-888

10.7503/cjcu20170548

Supported by the National Natural Science Foundation of China(Nos.41401358, 31472017), the China Postdoctoral Science Foundation(No.2013M542112) and the Natural Science Foundation of Hunan Province, China(No.2017JJ3100).

本校为第一且通讯机构

以芘作为外源荧光探针,采用牛血清蛋白作为参照,考察了转基因抗虫蛋白Cry1Ac与4种表面活性剂相互作用的荧光光谱特征.结果表明,鼠李糖脂和Tween 80体系的芘荧光行为有相似的变化规律,可与蛋白质发生稳定的缔合过程.Cry1Ac蛋白的亲水性和分子极性较强,在表面活性剂浓度低时,可导致芘的I1/I3值较高.在阴离子、非离子表面活性剂胶团形成后,2种蛋白质的存在均不改变芘与表面活性剂的结合位点.十六烷基三甲基溴化铵对芘的荧光有一定猝灭作用,且在Cry1Ac蛋白介入下不能形成稳定的胶束.

The characteristics of interactions between the transgenic insect-resistant protein(Cry1Ac) and four different surfactants were investigated by fluorescence of pyrene, an extrinsic fluorescent probe. While the bovine serum albumin(BSA) was used as a reference protein. The results show that the fluorescence behavior of pyrene in RL and Tween 80 systems has a similar change pattern, and the two surfactants can be associated with proteins stably. The hydrophily and molecular polarity of Cry1Ac protein are of high values, which lead to higher I1/I3...