National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41401358, 31472017]; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2013M542112]; Natural Science Foundation of Hunan Province, ChinaNatural Science Foundation of Hunan Province [2017JJ3100]
The characteristics of interactions between the transgenic insect-resistant protein(Cry1Ac) and four different surfactants were investigated by fluorescence of pyrene, an extrinsic fluorescent probe. While the bovine serum albumin(BSA) was used as a reference protein. The results show that the fluorescence behavior of pyrene in RL and Tween 80 systems has a similar change pattern, and the two surfactants can be associated with proteins stably. The hydrophily and molecular polarity of Cry1Ac protein are of high values, which lead to higher I1/I3 values of pyrene when the surfactant concentratio...
