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Establishment of regeneration system of Pyrus and the genetic stability analysis of regenerated population

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成果类型:
期刊论文
作者:
Liu, Qi;Yang, Yingjie;Liu, Jianlong;Song, Jiankun;Li, Dingli;...
通讯作者:
Rencai Wang<&wdkj&>Ran Wang
作者机构:
[Wang, Rencai; Liu, Qi] Hunan Agr Univ, Coll Hort, Changsha, Hunan, Peoples R China.
[Li, Dingli; Yang, Yingjie; Liu, Jianlong; Wang, Ran; Song, Jiankun] QingdaoAgr Univ, Qingdao Key Lab Genet Improvement & Breeding Hort, Qingdao, Shandong, Peoples R China.
通讯机构:
[Rencai Wang] C
[Ran Wang] Q
College of Horticulture, Hunan Agricultural University, Changsha, China<&wdkj&>Qingdao Key Lab of Genetic Improvement and Breeding of Horticultural Plants, QingdaoAgricultural University, Qingdao, China
语种:
英文
关键词:
Pyrus;Regeneration;Somaclonal variation;Genome resequencing
期刊:
Plant Cell, Tissue and Organ Culture
ISSN:
0167-6857
年:
2023
卷:
152
期:
1
页码:
215-228
基金类别:
This work was supported by the National Key R&D Program of China [2018YFD1000102]; China Agriculture Research System of MOF and MARA [CARS-28-07]; the Agricultural Variety Improvement Project of Shandong Province [2019LZGC008].
机构署名:
本校为第一机构
院系归属:
园艺园林学院
摘要:
Genetic assessment of pear regeneration population is very important for genetic fidelity maintenance in genetic engineering breeding. In this research, a regeneration system for a pear dwarfing rootstock cultivar, 'Qingzhen D1', was established using NN69 medium supplemented with different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA). The genetic stability of the regenerated population was evaluated through flow cytometry (FCM) and whole genome resequencing techniques. The highest regeneration rate (93%) was induced on NN69 medium supplemented with 3.0mg.L- 1 TDZ and 2....

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