Genetic assessment of pear regeneration population is very important for genetic fidelity maintenance in genetic engineering breeding. In this research, a regeneration system for a pear dwarfing rootstock cultivar, 'Qingzhen D1', was established using NN69 medium supplemented with different concentrations of thidiazuron (TDZ) and indole-3-butyric acid (IBA). The genetic stability of the regenerated population was evaluated through flow cytometry (FCM) and whole genome resequencing techniques. The highest regeneration rate (93%) was induced on NN69 medium supplemented with 3.0mg.L- 1 TDZ and 2....