期刊论文

Studies on determination by improved phenol-sulfuric acid method and extraction process of soluble polysaccharide from momordica charantia linn

中文

食品与机械

1003-5788

2007

23

4

72-75

10.13652/j.issn.1003-5788.2007.04.021

本校为其他机构

采用改良苯酚-硫酸法测定苦瓜及提取物中多糖的含量,以单糖组成比例组合而成的混合单糖为对照品制作糖的标准曲线,从而消除用葡萄糖做标准引起的系统误差,吸光度的变化与多糖含量呈线性关系,糖浓度在20～100 μg范围内线性较好,平均回收率达100.68%,其供试液在2 h内显色稳定.通过正交试验确定水溶性苦瓜多糖的最佳提取条件:浸提温度90℃,浸提时间为2 h,固液比1∶20,乙醇沉淀浓度为85%.苦瓜干粉样品中水溶性苦瓜多糖含量为8.20%.

A stable and simple method based on phenol-sulfuric reaction for the determination of hetero-polysaccharide was established. GC determined the proportion of sugars in the hetero-polysaccharide. According to standard solution obtained, heteropolysaccharide could be determined through standard curve. The calibration curve was linear （ R^2 = 0. 9968 ） in the range of 20 -100 μg for sugars. The average recovery of sugar was 100.68 %. GC and phenol-sulfuric acid method can be used to determine the heteropolysaccharides. The soluble polysaccharide...