期刊论文

中文

食品与机械

1003-5788

2014

30

2

81-83,166

10.3969/j.issn.1003-5788.2014.02.020

本校为第一机构

为解决目前发酵乳活菌总数测定过程中出现的菌体分散不均匀的问题,利用EDTA做螯合剂,通过对EDTA添加量以及稀释液pH值的优化,提供一种测定发酵乳活菌总数的改进方法。试验结果表明:EDTA的最佳添加量为2%~ 6%,发酵乳稀释液的最佳pH值范围为6.8~8.8。在最佳范围的试验条件下,发酵乳稀释液能在短时间内变澄清,并能防止菌体细胞受到高渗透压和高碱性环境的影响,保证了测定结果的准确性。测定结果与采用GB 4789.35-2010测定的结果接近,且精度要高于GB 4789.35-2010测定结果的精度。

To solve the problem that Lactic acid bacteria cells scattered unevenly in the determination of total viable count in fermented milk, EDTA was used as a chelating agent. An improved method for deter- mination of total viable count in fermented milk was provided, through the optimization of EDTA addition amount and pH value. The results showed that the optimal condition were： EDTA addition amount in the range of 2%-6%, the pH value in the range of 6.8~ 8.8. In the optimal ranges, fermented milk diluents could clarify in short time, the death of...