Acrylamide (AA) is a kind of neurotoxin and potential car cinogen, formed in heating food treatment, has been aroused exten- sive attention in all over the word. Accurate determination of AA in complex food system is the first importance to evaluate its harmful effects on human health. In this paper, the disadvantages of tradi- tional determination methods of AA, including liquid ehromatogra phy, gas chromotography and their coupling technique, were reviewed. Moreover, the complex of sample pretreatment process, re quirement of necessary samp...