期刊论文

中文

食品与机械

1003-5788

2019

35

4

42-47+119

10.13652/j.issn.1003-5788.2019.04.008

国家现代农业特色蔬菜产业技术体系专项(编号:CARS-24-E-02) 湖南省重点研发计划(编号:2016NK2110).

本校为第一机构

研究了2种硫代葡萄糖苷标准品质谱裂解规律,发现硫代葡萄糖苷的β-D-硫代葡糖、磺酸肟及来源于氨基酸侧链R上的键都能够断裂发生重排现象从而形成碎片离子。在质谱裂解规律的基础上对3种不同品种的花椰菜叶中硫代葡萄糖苷进行结构鉴定。鉴定结果表明,3种花椰菜叶中含有8种硫代葡萄糖苷,包括3-(甲基亚磺酰基)丙基硫代葡萄糖苷(1)、4-(甲基亚磺酰)丁基硫代葡萄糖苷(2)、3-丁烯基硫代葡萄糖苷(3)、4-羟基苄基硫代葡萄糖苷(4)、1-羟基-吲哚-3-亚甲基硫代葡萄糖苷(5)、3-吲哚甲基硫代葡萄糖苷(6)、1-含氧甲基吲哚-3-亚甲基硫代葡萄糖苷(7)、6-(甲基磺酰)己基硫代葡萄糖苷(8)。其中,宝塔花椰菜叶中含有...

A method for identification of glucosinolate in three kinds of cruciferous vegetable leaves was developed by using H P LC- Q-TOF- MS.Firstly, the mass spectrometry fragmentation pathways of two standard glucosinolates was studied.The bond of glucose, sulfated thiohydroximate and R-group was easily fractured and the rearrangement reaction was observed for the forming the fragment ions.Then, the structure of 8 glucosinolates including 3-( methylsulfinyl) propyl ( 1 ), 4-( methyl-sulfinyl) butyl (2), 3-butenyl ( 3 ), 4-hydroxybenzyl ( 4 ), 1- hydroxyindol-3-ylmethyl (5).Indol-3-ylmethyl (6).1-met...