期刊论文

Effects of polyphenols from Areca seed on intestinal microbes in vitro

中文

食品与机械

1003-5788

2019

35

1

41-46

10.13652/j.issn.1003-5788.2019.01.008

海南省重大科技计划项目(编号:ZDKJ2016003) 海南省应用技术研发与示范推广专项(编号:ZDXM2015013) 湖南省湘潭市重点项目(编号:NYZD20161002) 湘潭市科技特派员项目(编号:CXYTPZJ20171020).

本校为第一机构

通过间歇式静态厌氧体外发酵技术研究鲜槟榔籽中多酚类物质对青年健康男性肠道微生物的影响.采集24岁健康男性的粪便为菌源,分别以对照组(C组)、添加了纯度为74.09％的槟榔籽多酚的试验组(P组)为底物分别进行2,6,12,24h4个时间段的体外发酵.利用气相色谱、高通量测序等方法对pH值、多酚成分、短链脂肪酸(SCFAs)和微生物指标进行测定.结果 显示,槟榔籽多酚对青年健康男性肠道微环境中pH变化趋势影响不大,但会降低SCFAs的产量.肠道菌群方面,可以提高其肠道菌群的物种多样性水平,改变变形菌门(Proteobacteria)与厚壁菌门(Firmicutes)相对丰度比值,其中柔嫩梭菌属(Faecalibacterium)增加幅度最大,拟...

This study aimed to investigating the effects of the polyphenols from Areca seeds on young men’s intestinal microbes in vitro through intermittent static anaerobic fermentation technology.The feces of 24 years healthy young men are taken as starter.The substrates of the control group(group C)and the experimental group(group P)with a purity of 74.09% were fermented in vitro at 2,6,12 and 24 h.pH value,polyphenol composition,short chain fatty acid(SCFAs)and microorganism index were measured by gas chromatography and highthroughput sequencing.The...