期刊论文

中文

现代食品科技

1673-9078

2011

27

11

1344-1348

10.13982/j.mfst.1673-9078.2011.11.011

(2009FJ 1006-3):湖南省科技厅重大专项

本校为第一机构

本试验以菜籽粕为原料,从四种蛋白酶中挑选出中性蛋白酶和碱性蛋白酶对菜籽粕进行同步复合酶解.分别考察了不同的加酶量、酶比、料液比和时间对多肽得率的影响,并利用响应面分析法对混合蛋白酶水解菜籽粕的条件进行了优化.结果表明,在加酶量固定为4500 U/g,温度50℃,pH为8.0,料液比1∶8及碱性蛋白酶:中性蛋白酶为3∶1条件下酶解6.5 h,可使多肽得率达到54.89％.

In this paper,rapeseed meal was hydrolysed by alkaline protease and neutral protease.The relationship between the peptides yield and enzymatic hydrolysis conditions was studied.The reaction conditions catalyzed by the mixed proteases were optimized by the response surface methodology.The results showed that the optimizing hydrolysis condition was the enzyme dosage 4500 U/g,hydrolysis temperature 50 ℃,pH 8.0,the ratio of material to water 1：8,the ratio of alkaline protease to neutral protease 3：1,hydrolysis time 6.5 h.U...