期刊论文

中文

现代食品科技

1673-9078

2015

31

10

74-79

10.13982/j.mfst.1673-9078.2015.10.013

公益性行业（农业）科研专项（201303084）；

本校为第一机构

采用GB 4789.2-2010菌落总数计数及Miseq测序分析物种多样性及群落结构的方法,对蛋鸡场鸡蛋壳表面细菌数量及种群结构进行研究。结果检测8组样品的菌落总数为3.79±0.67~5.06±0.75 Log CFU/eggshell±SD;获得的163214条优化序列分属于变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、梭杆菌门(Fusobacteria)及蓝菌门(Cyanobacteria)。8组样品普遍存在假单胞菌属(Pseudomonas)、埃希氏-志贺氏菌属(Escherichia-Shigella)、泛菌属(Pantoea)、芽孢杆菌属(Bacillus)、肠球菌属(Enterococcus)细菌;而各样品分别以假单胞菌属(Pseudomonas)、埃...

The aerobic plate count (APC) and the bacterial population structure and diversity on the eggshell surface were studied on the basis of GB 4789.2-2010 and 16SrDNA sequence analysis using the Illumina Miseq Platform. The results showed that the APC values of eight samples ranged from 3.79±0.67 to 5.06±0.75 log CFU/eggshell±SD. The 163214 sequences of the eight samples belonged to Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Fusobacteria and Cyanobacteria, among which Enterococcus, Bacillus, Escherichia-Shigella, Pantoea, and Pseudomonas were dominant in eight ...