期刊论文

Yi, You-Jin

中文

现代食品科技

1673-9078

2015

31

5

76-83 and 162

10.13982/j.mfst.1673-9078.2015.5.013

2010RS4003:湖南省博士后科研资助专项 K1005013-31:长沙市科技局一般项目 2010079:湖南省中医药科研项目 2015BAD16B00:国家科技支撑计划 31071738:国家自然科学基金 31000827:国家自然科学基金

本校为第一且通讯机构

研究虎杖内生真菌对虎杖苷转化及转化产物抗氧化活性。采用薄层层析法筛选转化虎杖苷的内生真菌;通过形态学特征和ITS-5.8S r DNA基因序列分析对内生真菌进行鉴定;利用大孔吸附树脂、制备薄层色谱和高效液相色谱分离纯化生物转化产物;以VC、BHT、虎杖苷及5种虎杖苷衍生物为对照,对转化产物清除DPPH·自由基和OH·自由基的生物活性进行比较研究。经筛选发现其中l株内生真菌J3能转化虎杖苷形成转化产物。内生真菌J3 ITS-5.8S r DNA序列全长573bp,Gen Bank登录号KM 434883,确定为烟曲霉Aspergillus fumigatus。转化产物A-2在体外具有清除DPPH·自由基和OH·自由基的能力,且清除OH·自由基的能力高于...

The microbial transformation of polydatin by endophytic fungi isolated from Polygonum cuspidatum and the antioxidant activity of the transformed products were investigated. Screening of endophytic fungi for microbial transformation of polydatin was carried out using thin-layer chromatography (TLC). Fungi were identified by morphological characteristics and analysis of the ITS-5.8S rDNA region. Polydatin transformation products were isolated and purified by macroporous adsorption resin, preparative TLC, and high-performance liquid chromatography. Using vitamin C (Vc), BHT, polydatin, and five t...