期刊论文

Comparative Analysis of Antioxidant Activity of Crude Polysaccharides from Eurotium cristatum Ascospores

中文

现代食品科技

1673-9078

2019

35

9

102-109

10.13982/j.mfst.1673-9078.2019.9.012

黑茶金花湖南省重点实验室项目(2016TP1022) 湖南省重点研发计划资助项目(2018NK2036) 益阳市科技计划项目(2017YZ04)。

本校为第一机构

采用超微粉碎法、超声波法、反复冻融-研磨法,对冠突散囊菌子囊孢子进行破壁处理,探讨不同破壁方法对孢子粗多糖抗氧化活性的影响.结果 表明,破壁率由高至低依次为反复冻融-研磨法(93.73％)、超微粉碎法(82.27％)、超声波法(66.54％),粗多糖的提取率分别为2.18％、2.06％、1.84％,均显著高于未破壁孢子(1.15％).抗氧化试验表明,3种破壁孢子粗多糖均具一定抗氧化活性,且与质量浓度间存在着显著量效关系.以超微粉碎法破壁孢子粗多糖活性为最强,在其质量浓度为2.50 mg/mL时,对DPPH、OH、ABTS+三-种自由基清除能力分别为89.13％、95.22％、80.26％,铁离子还原能力为0.78.反复冻融-磨组和超声波组粗多...

The ultrafine grinding method, ultrasonic method, and repeated freeze-thaw and grinding method were used to break the wall of Eurotium cristatum ascospore. The effects of these wall-breaking methods on the antioxidant activity of crude polysaccharides from the ascospore were investigated. The results showed that the wall-breaking rate decreased in the order of the repeated freeze-thaw and grinding method (93.73%), the ultrafine grinding method (82.27%), and the ultrasonic method (66.54%), with their corresponding extraction rates for crude poly...