In this paper, the diversity distribution of kiwifruit soft rot diseases in main kiwifruit producing areas in Hunan Province was systematically analyzed. It provided basic data and theoretical basis for further study on the diversity of pathogenic fungi of kiwifruit soft rot in Hunan. Illumina HiSeq high-throughput sequencing technology was used to collect the second generation sequencing data of microbial ITS amplifiers from 15 groups of fruit samples of 5 soft-rotting kiwifruit (A, B, C, D, E), and bioinformatics analysis was performed. There...