期刊论文

Genetic Transformation System Establishment with Mature Internodal Stem Segments of Citrus Sinensis L.Osbeck cv. Bingtang

中文

经济林研究

1003-8981

2009

27

3

42-46

10.3969/j.issn.1003-8981.2009.03.009

:柑橘规模化转基因研究 (JY04-B-02):新品种选育

本校为第一机构

以柑橘成年态材料作为外植体进行遗传转化试验对遗传改良性状的早期评估是非常有利的,尤其是无核的品种如冰糖橙.本试验旨在建立一套冰糖橙成年态节间茎段再生及遗传转化体系,材料取自温室中嫁接于枳上的成年态植株.结果表明:新改良消毒处理可将外植体死亡率从27.2%降低为0,成活率从60%升高到80%左右;当采用MS+2ip 2 nag·L-1+2,4-D 2 mg·L-1共培培养基及MS+6-BA 3 mg·L-1再生培养基进行不定芽的诱导时,不定芽再生率最高为47.89%,在进行转化试验时,再生培养基需加入30 mg·L-1卡拉霉素和500 nag·L-1头孢霉素;采用3种方法将不定芽嫁接到14 d枳橙砧木上,其中微型嫁接中"改进的‘⊥'压法"可使嫁...

Citrus genetic transformation using mature tissue as explant is important for the precocious evaluation of the genetically modified characteristics, which is especially indispensable for seedless cuhivars like ＇Bingtang＇ orange. In this study, a protocol was improved for regeneration and genetic transformation with mature tissues of ＇Bingtang＇ orange. The internodal stem segments were sampled from adult plants grafted on Poncirus trifoliate and maintained in the greenhouse. The new improved disinfection could decrease the death rate from 27...