期刊论文

中文

经济林研究

1003-8981

2009

27

4

61-64

10.3969/j.issn.1003-8981.2009.04.013

本校为第一机构

采用新型分子标记SRAP技术对湖南省32份柚类及近缘种进行遗传多态性分析.筛选110对引物,选取稳定的12对引物扩增基因组DNA,各引物组合产生10～23条扩增带,共获得172条带,其中150条为多态性条带,每对引物平均提供12.5个标记信息.由UPGMA方法得到的聚类分析结果表明SRAP标记是一种能够较好的区分柚类种质的分子标记.

Genetic polymorphism of 32 Pummelo and its relatives were analyzed using new molecular marker SRAP. Twelve primers were selected from 110, which are steady on amplification. The average number of alleles per SRAP locus was 12.5 with a range from 10 to 23, totally 172 bands with 150 polymorphic bands. With the clustering result of UPGMA, it indicated that SRAP is a good molecu...