期刊论文

中文

经济林研究

1003-8981

2009

27

4

52-56

10.3969/j.issn.1003-8981.2009.04.011

湖南省重点课题（04JT） 湖南省教育厅学科带头人专项基金 湖南农业大学团队基金（04TD02） 湖南省财政厅课题.

本校为第一机构

为了建立豆梨子叶再生体系,以豆梨子叶为外植体,筛选出最适消毒方法后,接种在添加不同激素的培养基上。所有的培养基均附加7.5 g琼脂和30 g蔗糖,pH值为5.8,在温度（25±1）C、光强2 000 lx、光周期14 h/10 h的环境中进行培养,每30 d继代1次,来筛选最佳诱导培养基、分化培养基和生根培养基。结果表明,在1/4MS+6-BA 3 mg·L-1+NAA 0.2 mg·L-1培养基上外植体愈伤组织产生率为94.4%,在MS+6-BA 4 mg·L-1+NAA 0.2 mg·L-1培养基上不定梢诱导率达83.3%;长度为1 cm以上的不定梢在1/2MS+IBA 3 mg·L-1培养基上诱导出细长的丛生根,生根率87.5%。

In order to establish the regeneration system from cotyledon in P. calleryana Done, taking cotyledon in P. calleryana Dcne as materials, optimum callus inducing medium; differentiation medium and root inducing medium were selected. The materials were cultured in media supplemented with 7. 5 g/L agar, 30 g/L sucrose and different combinations of hormones, at the conditions of pH 5. 8, （25±1） C, alternation of 14 h light and 10 h dark, and 2 000 lx photosynthetic density, after the best sterilizing method was selected out. The results showed t...