期刊论文

中文

经济林研究

1003-8981

2009

27

3

1-6

10.3969/j.issn.1003-8981.2009.03.001

基金项目：广东省农科院资助项目（SR0273）.

本校为其他机构

以Me3和Em5正反向引物组合对凤凰水仙茶树品种进行了SRAP分析体系的优化.结果表明:茶树SRAP-PCR最佳反应体系为:Mgz+浓度3.0 mmol·L-1;Taq酶2.0 U;dNTPs浓度0.2 mmol·L-1;引物浓度2.0 μmol·L-1;10×Buffer 2.0μL;模板DNA 20 ng;反应总体积为25 μL.经过重复试验,确定茶树SRAP-PCR扩增程序为:94℃预变性5min;94C变性1min,35℃复性1min,72℃延伸100 s,5个循环;94C变性1 min,50℃复性1 min,72 C延伸100 s,35个循环·最后72℃延伸5 min.采用已优化的体系对30份茶树品种进行SRAP分析,经2%的琼脂糖凝胶电泳得到了清晰且重复性好的扩增谱带,说明该反应体系稳定可靠,适用于茶树种质资源的分子标记分...

An optimal system of SRAP analysis for Camellia sinensis（L.） O.Kuntze cultivar Fenghuang Shuixian was established with the primer combination of Me3 and Em5,the optimum reaction of which was composed of Mg2＋ 3.0 mmol·L-1,Taq 2.0 U,dNTPs 0.2 mmol·L-1,2.0 μmol·L-1 of each primer,10×Buffer 2.0 μL,20 ng DNA in a total volume of 25 μL.The optimized program was pre-denaturing at 94℃ for 5 min,then denaturing at 94℃ for 1 min,annealing at 35℃ for 1 min and extending at 72℃ for 100 s for the first five cycles; followed hy another 35 cycle...