期刊论文

The dynamic changing profiles of peripheral white blood cell telomere length in populations of different ages living at different altitude areas

Zhong, Y.C.

中文

中华预防医学杂志

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]

0253-9624

2008

42

7

502-505

10.3321/j.issn:0253-9624.2008.07.011

国家自然科学基金重大项目（30393130） 感谢青海大学医学院高原医学研究中心的王海洁、白振忠、马兰、杨应忠、韵海霞、曹越诸位老师对本课题的指导帮助,感谢青海省果洛藏族自治州花石峡卫生院,北京大学附属人民医院检验科提供样品

本校为其他机构

目的 探讨不同海拔地区不同年龄组人外周血白细胞端粒DNA长度变化.方法 分别选取高原组(花石峡海拔4380 m)与平原组(北京海拔43 m)健康人外周血标本172份.其中高原组95名(男性47名,女性48名),平原组77名(男性39名,女性38名).两组年龄分布基本相同.实验采用Southern杂交方法检测其端粒限制性片段长度(terminal restriction fragment,TRF),邻苯三酚氧化法测定超氧化物歧化酶(SOD),硫代巴比妥(TBA)比色法测定丙二醛(MDA)并做统计学分析.结果 高原组与平原组健康人平均TRF检测结果,高原组男性TRF值为10.45±1.35,女性为10.50±1.45;平原组男性TRF值为11.29±1.10,女性为11.31±1.13;高原组与平原组男...

OBJECTIVE: To investigate the relationship between the length of telomere DNA and age at different altitude areas. METHODS: All 172 peripheral blood samples were randomly selected from healthy individuals of different ages from 25 to 65 years old. High altitude group (47 males, 48 females) living at an altitude of 4380 m (HA group), sea level group (39 males, 38 females) living at an altitude of 43 m (SL group). The terminal restriction fragment (TRF) length of telomere DNA was measured by Southern blotting analysis. The plasma levels of malondialdehyde (MDA) and superoxide dismutase (SOD) wer...