期刊论文

Liu, HH

英文

Bioresource Technology

0960-8524

2024

406

130978

10.1016/j.biortech.2024.130978

National Natural Science Foundation of China [32060025]; Training Program for Excellent Young Innovators of Changsha [kq2305037]; Natural Science Foundation of Tibet Autonomous Region [XZ2019ZRG-79 (Z)]; Science and Technology Innovation Program of Hunan Province [2022RC1170]; Natural Science Foundation of Changsha [kq2208077]; Hunan Provincial Natural Science Foundation of China [2022JJ50022]; Foundation of State Key Laboratory of Utilization of Woody Oil Resource [GZKF202119]; Science and Technology Innovation Program of Forestry in Hunan Province [XLKY202310]; Hunan Agricultural University Graduate Student Research and Innovation Program [2023XC094]

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Cis -13, 16-docosadienoic acid (DDA) is an omega -6 polyunsaturated fatty acid with great potential for application in medicine and health. Using microbial cell factories for DDA production is considered a viable alternative to extracting DDA from plant seeds. In this study, using Yarrowia lipolytica Po1f ( Delta ku70) as a chassis, firstly, the adaptation of three elongases in Po1f ( Delta ku70) were explored. Secondly, the DDA biosynthetic pathway was redesigned, resulting in a DDA content of 0.046 % of total fatty acids (TFAs). Thirdly, through the " push-pull " strategy, the DDA content in...