期刊论文

Liu, HH

英文

Bioresource Technology

0960-8524

2024

406

130978

10.1016/j.biortech.2024.130978

Training Program for Excellent Young Innovators of Changsha, (kq2206037) Training Program for Excellent Young Innovators of Changsha Natural Science Foundation of Hunan Province, (2022JJ50022) Natural Science Foundation of Hunan Province Science and Technology Innovation Program of Forestry in Hunan Province, (XLKY202310) National Natural Science Foundation of China, NSFC, (kq2208077, 32060025) National Natural Science Foundation of China, NSFC Science and Technology Program of Hunan Province, (2022RC1170) Science and Technology Program of Hunan Province Natural Science Foundation of Tibet Autonomous Region, (XZ2019ZRG-79) Natural Science Foundation of Tibet Autonomous Region Foundation of State Key Laboratory of Utilization of Woody Oil Resource, (GZKF202119) Hunan Agricultural University Graduate Student Research and Innovation Program, (2023XC094) Hunan Science and Technology Talent Lifting Project - Young and Middle-Aged Excellent Science and Technology Talent Training Program, (2023TJ-Z04)

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Cis-13, 16-docosadienoic acid (DDA) is an omega-6 polyunsaturated fatty acid with great potential for application in medicine and health. Using microbial cell factories for DDA production is considered a viable alternative to extracting DDA from plant seeds. In this study, using Yarrowia lipolytica Po1f (Δku70) as a chassis, firstly, the adaptation of three elongases in Po1f (Δku70) were explored. Secondly, the DDA biosynthetic pathway was redesigned, resulting in a DDA content of 0.046 % of total fatty acids (TFAs). Thirdly, through the “pu...