A Novel Two-Dimensional Liquid Chromatography Combined with Ultraviolet Detection Method for Quantitative Determination of Pyridoxal 5′-Phosphate, 4-Pyridoxine Acid and Pyridoxal in Animal Plasma

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成果类型：

期刊论文

作者：

Yang, Rong-Ju;Wang, Na;Ma, Xiao;Gong, Meng-Die;Wang, Yi-Rong;...

通讯作者：

Tang, Q.;Liu, Z.-Y.

作者机构：

[Yang, Rong-Ju; Wang, Yi-Rong; Gong, Meng-Die; Liu, Zhao-Ying; Wang, Na; Meng, Si-Yu; Ma, Xiao] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Peoples R China.

[Yang, Rong-Ju; Wang, Yi-Rong; Gong, Meng-Die; Liu, Zhao-Ying; Wang, Na; Meng, Si-Yu; Ma, Xiao] Hunan Agr Univ, Hunan Engn Technol Res Ctr Vet Drugs, Changsha 410128, Peoples R China.

[Tang, Qi] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.

通讯机构：

[Zhao-Ying Liu; Qi Tang] A

Authors to whom correspondence should be addressed.<&wdkj&>College of Horticulture, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China<&wdkj&>Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China

语种：

英文

关键词：

4-pyridoxic acid;pyridoxal;pyridoxal 5′-phosphate;two-dimensional liquid chromatography;vitamin B6

期刊：

ANIMALS

ISSN：

2076-2615

年：

2023

卷：

期：

页码：

1333-

DOI：

10.3390/ani13081333

基金类别：

This work was supported by the National Natural Science Foundation of China (No. 31972737).

机构署名：

本校为第一机构

院系归属：

园艺园林学院

动物医学院

摘要：

Vitamin B6 is an indispensable micronutrient in organisms and is widely distributed in blood, tissues, and organs. Changes in the content and ratio of vitamin B6 can affect the entire physiological condition of the body, so it becomes particularly important to reveal the relationship between changes in its content and disease by monitoring vitamin B6 levels in the organism. In this study, a two-dimensional liquid chromatography-UV detector (2D-LC-UV) was used to establish a method for the simultaneous detection of PLP, PA, and PL for the first ...

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A Novel Two-Dimensional Liquid Chromatography Combined with Ultraviolet Detection Method for Quantitative Determination of Pyridoxal 5′-Phosphate, 4-Pyridoxine Acid and Pyridoxal in Animal Plasma

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