期刊论文

中文

食品研究与开发

1005-6521

2015

8

34-39

10.3969/j.issn.1005-6521.2015.08.010

基金项目：国家星火计划重大项目（2011GA770007） 湖南省重大科技专项（2010FJ1007-2）

本校为第一机构

采用酸法、碱法、酶法、水法从鱼皮中提取胶原蛋白，对其性质进行分析比较。结果表明，提取率，酸法＞酶法＞碱法＞水法；热变性温度，水法＞酶法＞碱法＞酸法；水法和碱法提取的胶原蛋白SDS-PAGE电泳图谱条带中出现β二聚体和γ三聚体，酶法和酸法没有出现，但是酶法和酸法的α链位置明显色带浓于水法和碱法；紫外吸收光谱中，酸法和碱法的吸收峰强度大于水法和酶法，碱法和酶法左侧出现了杂峰；红外光谱显示，碱法提取的鱼皮胶原蛋白不具备酰胺带特征吸收峰，其二级结构已破坏，酸法、酶法和水法提取的胶原蛋白均存在酰胺带特征吸收峰，而且出现位置相似，符合胶原蛋白特征吸收峰。

Extract collagen of grass carp's skin with acid, alkali ,enzyme and water method, the result of collagen's extraction yield in different method was as follow:acid>alkali>enzyme>water. The result of collagen's in different method was as follow: alkali >acid >water >enzyme. The result of collagen's SDS -PAGE electrophoresis was: the ribbons from water and alkali appeared in β-dipolymer and γ-tripolymer ribbons position, the ribbons from enzyme and acid no appearance, butα-chain's ribbons from enzyme and acid marks significantly thicker than ri...