期刊论文

中文

草业学报

1004-5759

2007

16

5

121-126

10.3321/j.issn:1004-5759.2007.05.017

本校为第一机构

以匐匍翦股颖L-93的胚性愈伤为试验材料,用适宜剂量为20 Gy的60CoY射线进行辐射处理,将经辐照处理的胚性愈伤接种在含有PEG6000浓度为200 g/L的NB筛选培养基中进行抗旱筛选,获得耐旱再生植株,并对这些植株进行形态学以及干旱胁迫后植物的游离脯氨酸(Pro)的积累、叶绿素含量、细胞膜相对透性以及过氧化物酶(POD)、超氧物歧化酶(SOD)、过氧化氢酶(CAT)活性等方面进行抗旱性鉴定.结果表明,变异体植株的抗旱性明显强于对照.

Embryogenic callus of Agrostis stolonifera L-93 was treated with 20 Gy ^60Co gamma-ray and cultured in NB selective medium containing 200 g/L of PEG6000 and drought-resistant regenerated plants were obtained. Such plants were identified on morphology and on drought-tolerance from the Pro accumulation, chlorophyll content, cell membrane relative penetrability, peroxidase （POD）, superoxide dismutase （SOD）, and catalase （CAT） activity after drought-stress. The results indicated that the drought-t...