期刊论文

中文

湖南农业大学学报(自然科学版)

1007-1032

1998

24

2

137-142

10.13331/j.cnki.jhau.1998.02.012

湖南省科委资助；

本校为第一机构

以草鱼和鲤为材料,提取基因组DNA,制备RAPD-PCR模板,对3个随机引物盒共60个10-mer引物进行了PCR扩增,共筛选出7个较理想的可用于鱼种内或鱼种间群体遗传分析的随机引物。用这7个引物所获得的草鱼和鲤的RAPD指纹图谱平均带纹总数分别为4.28±1.89和6.71±4.19,多态性带纹总数分别为1.71±1.60和5.14±4.74。RAPD指纹图谱特征反映出草鱼比鲤具有更高的遗传纯度。7个引物共扩增出草鱼特异性带纹18条,鲤特异性带纹32条。大量特异性带纹的检出表明,两个鱼种间存在较大的遗传差异,同时也为鱼种间基因转移(特别是全基因组DNA导入)提供了分子检测的候选遗传标记。

Genomic DNA was extracted as RAPD PCR template from the liver tissue of grass carp and common carp, 60 10 mer random primers were used and 7 primers were proved to be ideal for population genetic analysis within and or between the fish species. The average number of total RAPD bands amplified with these 7 primers for grass carp and common carp were 4 28±1 89 and 6 71±4 19,respec tively, the average number of polymorphic bands were 1.71±1.60 and 5.14±4.74, respectively. The RAPD patterns suggested that the grass carp have higher genetic homo...