期刊论文

Tian, Yun;Ji, Xiao-Jun

英文

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

0021-8561

2019

67

35

9851-9857

10.1021/acs.jafc.9b03727

This work was supported by the National Science Fund for Excellent Young Scholars of China (no. 21922806), the National Natural Science Foundation of China (Nos. 21808052, 21776131, and 21476111), the Science and Technology Department of Hunan Province (no. 2018RS3086), the Scientific Research Fund of Hunan Provincial Education Department (no. 18B090), and the Double first-class construction project of Hunan Agricultural University (no. SYL201802002).

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Arachidonic acid (ARA, C20:4) is a typical ω-6 polyunsaturated fatty acid with special functions. Using Yarrowia lipolytica as an unconventional chassis, we previously showed the performance of the Î""-6 pathway in ARA production. However, a significant increase in the Î""-9 pathway has rarely been reported. Herein, the Î""-9 pathway from Isochrysis galbana was constructed via pathway engineering, allowing us to synthesize ARA at 91.5 mg L-1. To further improve the ARA titer, novel enzyme fusions of Î""-9 elongase and Î""-8 desaturase wer...