Arachidonic acid (ARA, C20:4) is a typical ω-6 polyunsaturated fatty acid with special functions. Using Yarrowia lipolytica as an unconventional chassis, we previously showed the performance of the Î"-6 pathway in ARA production. However, a significant increase in the Î"-9 pathway has rarely been reported. Herein, the Î"-9 pathway from Isochrysis galbana was constructed via pathway engineering, allowing us to synthesize ARA at 91.5 mg L-1. To further improve the ARA titer, novel enzyme fusions of Î"-9 elongase and Î"-8 desaturase were red...
