期刊论文

Huang, Ke

英文

BMC Genomics

1471-2164

2019

20

1

1-13

10.1186/s12864-019-5758-2

This work was supported byNational Natural Science Foundation of China (No. 31772325), Hunan Provincial Natural Science Foundation of China (2017JJ2118,2018JJ3217), Hunan Provincial Sci-Tech Project (2018NK2022) and Scientific Research Fund of Hunan Provincial Education Department (16A093). The funders did not have any role in the design, collection, analysis, and interpretation of data and in writing the manuscript.

本校为第一且通讯机构

BackgroundSulforaphane is a natural isothiocyanate available from cruciferous vegetables with multiple characteristics including antioxidant, antitumor and anti-inflammatory effect. Single-molecule real-time (SMRT) sequencing has been used for long-read de novo assembly of plant genome. Here, we investigated the molecular mechanism related to glucosinolates biosynthesis in Chinese kale using combined NGS and SMRT sequencing.ResultsSMRT sequencing produced 185,134 unigenes, higher than 129,325 in next-generation sequencing (NGS). NaCl (75mM), methyl jasmonate (MeJA, 40M), selenate (Se, sodium s...