期刊论文

STUDIES OF BLACK-SPOT DISEASE ON ROSE

中文

湖南农业大学学报(自然科学版)

1007-1032

1990

02

160-168

10.13331/j.cnki.jhau.1990.02.009

本校为第一机构

1986～1988年,以PDA加500 ppm五氯硝基苯的组织分离法和取病斑上分生孢子分离法,分离月季黑斑病菌获得成功,病菌在PDA和燕麦培养基上生长良好,果糖和甘露醇促进孢子发芽率;病菌菌丝生长和产孢温度范围为10～35℃,最适温度22～25℃,最适pH 6;碳源为棉子糖和菊糖,氮源为L-丙氨酸和L-异亮氨酸最好,长沙地区月季黑斑病周年发生,每年5,6月和10月下旬至12月上旬为发病高峰期,采用配制土栽培,在施用2%尿素水或波美5度人粪水时,结合喷施0.1%四硼酸钠或0.1%硫酸锌微肥,既能防病又能提高观赏价值,药剂防治以施用75%百菌清500倍和18%多菌铜300倍液,每隔15～20 d喷1次,连喷2或3次,可控制病害。

Black-spot disease (Diplocarpon rosae wolf) has been success-fully isolated with tissue isolation from the culture medium of PDA+500 ppmpentachlore-nitrobenzene and with single-spore isolation from spores on lesion.Pathogenes grow well on PDA and oat dextrose agar culture medium. D-fructoseand mannite increase the germination rate of spores. Pathogen developing andspore forming occur during the temperature of 10~35℃, with 22~25℃ optimal.The optimal acidity is pH 6. The best carbon sources are raffinose and inulin,and nitrogen L-alanine and L-...