从稗叶枯病株分离病原菌(Helminthosporium monoceras D rechsler)11个菌株,其培养滤液均可抑制稗的胚根生长,与对照比胚根平均相对生长率为28.47%~53.60%,适于菌株的生长和毒素产生的培养液为10%稗汁葡萄糖和改良Fires培养液,适于菌株生长和产毒的温度为25~30℃,pH 6~7.通气有利于菌株的生长,但对产毒无显著影响,连续光照或连续黑暗有利于菌株产毒,菌株在pH 6~7的PDA培养基上,于30℃连续黑暗的培养产孢量最多。
摘要(英文):
Culture filtrates of 11 isolates separated from diseased plants withHelminthosporium monoceras Drechsler have been found inhibiting the radiclegrowth of barnyard-grass.The relative radicle growth ratio of the seeds treatedranged 28.47~53.60%.The modified Fires medium and glucose broth plus 10%barnyard-grass juice is best for growth and toxin production of the pathogen.The optimum temperature is 25~30℃ and the pH value 6~7.Aeration provedbeneficial to growth of the pathogen but insignificant to toxin production.Continuous lighting or darkening ...